Appleton takes over the captaincy from Nicolas Martins, who was part of the match-day squad that beat Namibia by 37-22.
Martins is replaced on the side of the scrum by Diego Pinheiro, with Vasco Baptista taking over from Joao Granate at No 8. At lock, Nicolas Fernandes replaces Antonio Rebelo Andrade, who moves to the bench.
Mannix has made a few tweaks to the backline too, with Joris Moura starting at flyhalf and Simao Bento at fullback, while Manuel Cardoso Pinto will run out at wing after donning the No 15 jersey last week.
Portugal team:
15 Simao Bento
14 Manuel Cardoso Pinto
13 Jose Lima
12 Tomas Appleton (captain)
11 Rodrigo Marta
10 Joris Moura
9 Hugo Camacho
8 Vasco Baptista
7 Diego Pinheiro
6 Jose Maderia
5 Duarte Torgal
4 Nicolas Fernandes
3 Diogo Hasse Ferreira
2 Luka Begic
1 Francisco Fernandes
Replacements:
16 David Costa
17 Pedro Vicente
18 Abel Cunha
19 Antionio R. Andrade
20 Andre Cunha
21 Pedro Lucas
22 Domingos Cabral
23 Jose P. Santos
